At TIPS journal club meeting held on January 14, 2019, the topic of “Effects of needle size on inflammation during cecal legation and puncture” was presented by T. Didari. A summary of this presentation is in below.
Sepsis as a result of host and pathogens interaction causes the organ damage and plays a critical role for wide range of intensive care unit (ICU) and emergency department (ED) patients. This study is designed to evaluate the outcome of the CLP procedure in murine models using two routine needle sizes, 18 and 21. To this end, rats were randomly assigned into CLP procedure with gauge 18, 21, and the sham. Oxidative stress markers, blood markers, apoptosis indicators, and gene expression of autophagy and cellular hypoxia were measured in all animals. Besides, histopathological assessments were carried out and the levels of inflammatory cytokines such as (TNF-α) and (HMGB1) were measured in both cardiac and pulmonary tissues 24 hours after the procedure. The results indicated that although all measured factors increased in both 18 and 21 gauge groups in comparison with the shams, inflammatory markers, blood markers, blood and tissue lactate levels, pro-inflammatory cytokines, 3 and 9 caspases, gene expression of cellular homeostasis and autophagy in tissue samples were most prominently increased in the gauge 18 group in comparison with the gauge 21 ones 24 hours following the procedure. Our findings indicate that in murine models of sepsis induced by CLP procedure, gauge size can be influential in the outcomes. More specifically, using gage 18 needles leads to superior results in comparison with gage 21ones.